Less and shrunken pollen 1 (LSP1) encodes a member of the ABC transporter family required for pollen wall development in rice (Oryza sativa L.)
Tao Luo,
Ting Zou,
Guoqiang Yuan,
Zhiyuan He,
Wenjie Li,
Yang Tao,
Miaomiao Liu,
Dan Zhou,
Hongfeng Zhao,
Jun Zhu,
Yueyang Liang,
Qiming Deng,
Shiquan Wang,
Aiping Zheng,
Huainian Liu,
Lingxia Wang,
Ping Li,
Shuangcheng Li
Affiliations
Tao Luo
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Ting Zou
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Guoqiang Yuan
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Zhiyuan He
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Wenjie Li
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yang Tao
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Miaomiao Liu
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Dan Zhou
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Hongfeng Zhao
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Jun Zhu
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yueyang Liang
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Qiming Deng
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Shiquan Wang
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Aiping Zheng
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Huainian Liu
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Lingxia Wang
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Ping Li
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; State Key Laboratory of Hybrid Rice, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Shuangcheng Li
State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; State Key Laboratory of Hybrid Rice, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Corresponding author at: State Key Laboratory of Crop Genetic Resource Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Pollen fertility is an agronomic trait that strongly influences rice yield. Recent studies have revealed that the development of the pollen wall is required for pollen fertility and is regulated by several genes. However, the mechanisms underlying pollen and pollen wall development in rice remain largely unknown. In the present study, a point mutation in a gene on chromosome 1 was identified that resulted in the production of less and shrunken pollen (LSP) and led to defects in pollen wall formation. This gene was named LSP1 and was found to encode a member of the adenosine triphosphate-binding cassette (ABC) transporter G subfamily, OsABCG3. Two other loss-of-function mutants of LSP1/OsABCG3, generated using CRISPR/Cas9 technology, showed the same male sterile phenotype. The LSP1/OsABCG3 gene showed a spatio-temporal expression pattern in the developing anthers, and is an ortholog of the Arabidopsis genes AtABCG1 and AtABCG16, which play an important role in pollen wall development. Mutation of LSP1/OsABCG3 affected the expression of several genes involved in pollen and pollen wall formation. These results suggest that LSP1/OsABCG3 is critical for normal pollen fertility and shed light on the molecular mechanisms underlying rice pollen wall development.
