Nature Communications (Mar 2021)

Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes

  • Shirley Tremel,
  • Yohei Ohashi,
  • Dustin R. Morado,
  • Jessie Bertram,
  • Olga Perisic,
  • Laura T. L. Brandt,
  • Marie-Kristin von Wrisberg,
  • Zhuo A. Chen,
  • Sarah L. Maslen,
  • Oleksiy Kovtun,
  • Mark Skehel,
  • Juri Rappsilber,
  • Kathrin Lang,
  • Sean Munro,
  • John A. G. Briggs,
  • Roger L. Williams

DOI
https://doi.org/10.1038/s41467-021-21695-2
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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The phosphatidylinositol-3-phosphate (PI3P) is generated by the lipid kinase VPS34, in the context of VPS34 complex I on autophagosomes or complex II on endosomes. Biochemical and structural analyses provide insights into the mechanism of both VPS34 complexes recruitment to and activation on membranes by specific Rab GTPases.