Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

Faithful Makita-Chingombe; Anthony T. Podany; Timothy Mykris; Farai Muzambi; Richard W. Browne; Andrew J. Ocque; Robin DiFrancesco; Lee C. Winchester; Courtney V. Fletcher; Tinashe Mudzviti; Charles C. Maponga; Gene D. Morse

doi:10.4102/ajlm.v10i1.1264

African Journal of Laboratory Medicine (Jul 2021)

Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

Faithful Makita-Chingombe,
Anthony T. Podany,
Timothy Mykris,
Farai Muzambi,
Richard W. Browne,
Andrew J. Ocque,
Robin DiFrancesco,
Lee C. Winchester,
Courtney V. Fletcher,
Tinashe Mudzviti,
Charles C. Maponga,
Gene D. Morse

Affiliations

Faithful Makita-Chingombe: International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare
Anthony T. Podany: Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska
Timothy Mykris: Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska
Farai Muzambi: International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare
Richard W. Browne: Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York
Andrew J. Ocque: Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York
Robin DiFrancesco: Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York
Lee C. Winchester: Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska
Courtney V. Fletcher: Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska
Tinashe Mudzviti: International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare
Charles C. Maponga: International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe; and, Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York
Gene D. Morse: Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York

DOI: https://doi.org/10.4102/ajlm.v10i1.1264
Journal volume & issue: Vol. 10, no. 1
pp. e1 – e5

Abstract

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An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

Published in African Journal of Laboratory Medicine

ISSN: 2225-2002 (Print); 2225-2010 (Online)
Publisher: AOSIS
Country of publisher: South Africa
LCC subjects: Medicine: Public aspects of medicine; Medicine: Medicine (General)
Website: https://ajlmonline.org/index.php/ajlm

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Abstract

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