Functionalization of MWCNTs for Bioelectrocatalysis by Bacterial Two-Domain Laccase from <i>Catenuloplanes japonicus</i>
Azat Abdullatypov,
Pavel Oskin,
Veronika Fedina,
Liubov Trubitsina,
Sofiya Yakimovich,
Ekaterina Shuvalova,
Pradeep Verma,
Tatyana Dyachkova,
Olga Ponamoreva,
Sergey Alferov
Affiliations
Azat Abdullatypov
Biotechnology Department, Tula State University, Pr. Lenina 92, 300012 Tula, Russia
Pavel Oskin
Laboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, Russia
Veronika Fedina
Laboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, Russia
Liubov Trubitsina
Institute of Biochemistry and Physiology of Microorganisms RAS—A Separate Subdivision of Federal Research Centre Puschino Scientific Center for Biological Research RAS, Prospekt Nauki, 5, 142290 Pushchino, Russia
Sofiya Yakimovich
Laboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, Russia
Ekaterina Shuvalova
Biotechnology Department, Tula State University, Pr. Lenina 92, 300012 Tula, Russia
Pradeep Verma
Department of Microbiology, Central University of Rajasthan Bandar Sindri, 613 E Haribhau Upadhaya Nagar, Ajmer 305817, India
Tatyana Dyachkova
Department of Technology and Methods of Nanoproducts Manufacturing, Tambov State Technical University, 106/5, Building 2, Sovetskaya Str., 392000 Tambov, Russia
Olga Ponamoreva
Laboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, Russia
Sergey Alferov
Biotechnology Department, Tula State University, Pr. Lenina 92, 300012 Tula, Russia
This study was carried out in order to assess several modifications of carbon nanotube-based nanomaterials for their applications in laccase electrodes and model biofuel cells. The modified MWCNTs served as adapters for the immobilization of laccase from Catenuloplanes japonicus VKM Ac-875 on the surface of electrodes made of graphite rods and graphite paste. The electrochemical properties of the electrodes were tested in linear and cyclic voltammetrical measurements for the determination of the redox potential of the enzyme and achievable current densities. The redox potential of the enzyme was above 500 mV versus NHE, while the highest current densities reached hundreds of µA/cm2. Model biofuel cells on the base of the laccase cathodes had maximal power values from 0.4 to 2 µW. The possibility of practical application of such BFCs was discussed.
