Tandem fluorescent Halo-GFP reporter for quantitative and time-resolved monitoring of organelle and protein delivery to lysosomes

Abstract

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Keywords

• alpha-1 antitrypsin z variant (atz)
• autophagy
• endolysosomes
• endoplasmic reticulum autophagy (er-phagy/reticulophagy)
• er-to-lysosome-associated degradation (erlad)
• recov-er-phagy
• quantitative and time-resolved assays
• tandem fluorescent reporter