Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples

Abstract

Summary: The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health and disease. We describe a protocol for detection of glycan-degrading enzyme activity in mouse and human fecal samples, namely sulfatase and four carbohydrate-active enzymes. Assessing their activity can inform treatment strategies for diseases linked to the gut microbiome.For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).

Keywords

• Metabolism
• Protein expression and purification