Clinical Interventions in Aging (May 2019)
Changes in the blood antioxidant defense of advanced age people
Abstract
Mariusz Kozakiewicz,1 Maciej Kornatowski,2 Olga Krzywińska,1 Kornelia Kędziora-Kornatowska21Nicolaus Copernicus University in Torun Ludwig Rydygier Collegium Medicum in Bydgoszcz, Department of Food Chemistry, Bydgoszcz, Poland; 2Nicolaus Copernicus University in Torun Ludwig Rydygier Collegium Medicum in Bydgoszcz, Department and Clinic of Geriatrics, Bydgoszcz, PolandIntroduction: Since 1956 there have been numerous scientific articles about free radical theory of aging which both confirm and deny the theory. Due to oxygen metabolism, there are relatively high concentrations of molecular oxygen in human cells, especially in mitochondria. Under normal physiological conditions, a small fraction of oxygen is constantly converted to ROS, such as superoxide radical (O2−•,), H2O2, and related metabolites.Aim of the study: The aim of this work was to show the relation between the activity of main antioxidative enzymes and the age of the examined patients.Materials and methods: The analysis of antioxidant defense was performed on the blood samples from 184 “aged“ individuals aged 65–90+ years, and compared to the blood samples of 37 individuals just about at the beginning of aging, aged 55–59 years.Results: The statistically significant decreases of Zn,Cu-superoxide dismutase (SOD-1), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were observed in elderly people in comparison with the control group. Moreover, an inverse correlation between the activities of SOD-1, CAT, and GSH-Px and the age of the examined persons was found. No age-related changes in glutathione reductase activities and malondialdehyde concentrations were observed.Conclusion: Lower activities of fundamental antioxidant enzymes in the erythrocytes of elderly people, which indicate the impairment of antioxidant defense in the aging organism and the intensity of peroxidative lipid structures, were observed.Keywords: aging, antioxidant enzymes, free radicals, oxidative stress
