CHIMIA (Nov 2010)

IMAPlate Based Miniature, High Sensitive, Rapid Screening Method for Detecting Bioengineered, Secreted Lipase Activities in Yeast Expression Systems

  • Michel A. Sciotti,
  • Guo Jun Chen,
  • Daniel Gygax

DOI
https://doi.org/10.2533/chimia.2010.789
Journal volume & issue
Vol. 64, no. 11

Abstract

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A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances.

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