Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating
Leonardo P. Faverani,
William P. P. Silva,
Cecília Alves de Sousa,
Gileade Freitas,
Ana Paula F. Bassi,
Jamil A. Shibli,
Valentim A. R. Barão,
Adalberto L. Rosa,
Cortino Sukotjo,
Wirley G. Assunção
Affiliations
Leonardo P. Faverani
Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery and Implantology, School of Dentistry, São Paulo State University—UNESP, Araçatuba 16015-050, Brazil
William P. P. Silva
Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery and Implantology, School of Dentistry, São Paulo State University—UNESP, Araçatuba 16015-050, Brazil
Cecília Alves de Sousa
Dental Prosthesis, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University—UNESP, Araçatuba 16015-050, Brazil
Gileade Freitas
Bone Res Lab, School of Dentistry of Ribeirao Preto, University of São Paulo (USP), Ribeirão Preto 14040-904, Brazil
Ana Paula F. Bassi
Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery and Implantology, School of Dentistry, São Paulo State University—UNESP, Araçatuba 16015-050, Brazil
Jamil A. Shibli
Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos (UnG), Guarulhos 07023-070, Brazil
Valentim A. R. Barão
Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba 13414-903, Brazil
Adalberto L. Rosa
Bone Res Lab, School of Dentistry of Ribeirao Preto, University of São Paulo (USP), Ribeirão Preto 14040-904, Brazil
Cortino Sukotjo
Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA
Wirley G. Assunção
Dental Prosthesis, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University—UNESP, Araçatuba 16015-050, Brazil
Chemical and topographical surface modifications on dental implants aim to increase the bone surface contact area of the implant and improve osseointegration. This study analyzed the cellular response of undifferentiated mesenchymal stem cells (MSC), derived from senile rats’ femoral bone marrow, when cultured on a bioactive coating (by plasma electrolytic oxidation, PEO, with Ca2+ and P5+ ions), a sandblasting followed by acid-etching (SLA) surface, and a machined surface (MSU). A total of 102 Ti-6Al-4V discs were divided into three groups (n = 34). The surface chemistry was analyzed by energy dispersive spectroscopy (EDS). Cell viability assay, gene expression of osteoblastic markers, and mineralized matrix formation were investigated. The cell growth and viability results were higher for PEO vs. MSU surface (p = 0.001). An increase in cell proliferation from 3 to 7 days (p p p = 0.001) among groups. In addition, the PEO surface showed a higher mineralized matrix bone formation (p = 0.003). In conclusion, MSC from senile female rats cultured on SLA and PEO surfaces showed similar cellular responses and should be considered for future clinical investigations.
