The role of Cockayne Syndrome Protein B in transcription regulation

Abstract

We investigated the question if CSB (Cockayne Syndrome complementation B) protein actively regulates gene transcription and how mutations in CSB gene affect that regulatory role. Here we describe how we processed and interpreted ChIP-seq data (deposited in Gene Expression Omnibus with accession number GSE50171) obtained during an investigation of that question, and how this analysis assisted in the generation of hypothesis that were subsequently validated using other types of experiment.

Keywords

• Cockayne Syndrome
• Regulation of gene
• expression
• Enhancers
• Promoters
• Motif analysis