Infection and Drug Resistance (Aug 2022)
Analysis of the Value of Serum Biomarker LBP in the Diagnosis of Spinal Tuberculosis
Abstract
Caili Lou,1,2 Jian Liu,2 Zhibo Ren,2 Jinke Ji,2 Hongbao Ma,2 Hui Dong,3 Linan Wang,2 Xu Zhang,3 Ningkui Niu1 1Department of Spinal Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, People’s Republic of China; 2Clinical Medical School, Ningxia Medical University, Yinchuan, Ningxia, 750004, People’s Republic of China; 3Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, People’s Republic of ChinaCorrespondence: Ningkui Niu, Department of Spinal Surgery, General Hospital of Ningxia Medical University, No. 804 Shengli Street, Yinchuan, 750004, People’s Republic of China, Tel +86 13629593280, Email [email protected] Xu Zhang, Institute of Medical Sciences, General Hospital of Ningxia Medical University, No. 804 Shengli Street, Yinchuan, Ningxia, 750004, People’s Republic of China, Email [email protected]: To investigate the correlation between the expression of lipopolysaccharide-binding protein (LBP) in peripheral blood of spinal tuberculosis and clinical diagnosis and to evaluate its value as a diagnostic marker of spinal tuberculosis.Methods: In the experimental group, clinical history data and peripheral blood were collected from 100 patients with spinal tuberculosis who were admitted to the Department of Spine Surgery, General Hospital of Ningxia Medical University from May 2017 to May 2020, and peripheral blood was collected from 30 healthy volunteers in the control group. Screening of differential LBP expression by proteomics and ELISA to verify its expression in peripheral blood of spinal tuberculosis patients. t-test, Spearman analysis, linear regression and ROC curve were used to evaluate the diagnostic value of LBP in peripheral blood for spinal tuberculosis.Results: The expression of LBP protein in peripheral blood is significantly higher in patients with spinal tuberculosis than in the normal population; LBP assay values were significantly and positively correlated with CRP and ESR values (P < 0.01); the AUC of LBP in the diagnosis of spinal tuberculosis for pathological examination, bacteriological culture, T-cell spot test for tuberculosis infection (T-SPOT), imaging diagnosis, and acid fast bacillus were, respectively, 0.677 (P < 0.01), 0.707 (P < 0.01), 0.751 (P < 0.01), 0.714 (P < 0.01), and 0.656 (P < 0.05), and there was a correlation between LBP and the diagnostic evaluation of spinal tuberculosis.Conclusion: LBP could be a new candidate biomarker for the diagnosis of spinal tuberculosis.Keywords: spinal tuberculosis, proteomics, LBP, biomarker
