Detection of <i>Vibrio parahaemolyticus</i> Based on Magnetic and Upconversion Nanoparticles Combined with Aptamers
Xinjie Song,
Wei Li,
Li Wu,
Tianfeng Lv,
Yao Zhang,
Juan Sun,
Xuping Shentu,
Xiaoping Yu,
Yuanfeng Wu
Affiliations
Xinjie Song
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, China
Wei Li
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Republic of Korea
Li Wu
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, China
Tianfeng Lv
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, China
Yao Zhang
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, China
Juan Sun
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, China
Xuping Shentu
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou 314423, China
Xiaoping Yu
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou 314423, China
Yuanfeng Wu
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, China
Vibrio parahaemolyticus is a halophilic and heat-labile gram-negative bacterium and is the most prevalent foodborne bacterium in seafood. In order to develop a rapid and sensitive method for detecting the foodborne pathogenic bacterium Vibrio parahaemolyticus, an aptamer-modified magnetic nanoparticle and an aptamer-modified upconversion nanoparticle were synthesised and used as a capture probe and a signal probe, respectively. The aptamer-modified magnetic nanoparticle, V. parahaemolyticus cell, and aptamer-modified upconversion nanoparticle formed a sandwich-like complex, which was rapidly separated from a complex matrix using a magnetic force, and the bacterial concentration was determined by fluorescence intensity analysis. The results showed that the fluorescence intensity signal correlated positively with the concentration of V. parahaemolyticus in the range of 3.2 × 102 to 3.2 × 105 CFU/mL, with a linear equation of y = 296.40x − 217.67 and a correlation coefficient of R2 = 0.9610. The detection limit of the developed method was 4.4 CFU/mL. There was no cross-reactivity with other tested foodborne pathogens. This method is highly specific and sensitive for the detection of V. parahaemolyticus, and can achieve the qualitative detection of this bacterium in a complex matrix.
