Nature Communications (Nov 2020)

The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations

  • Junqiao Jia,
  • Eva Absmeier,
  • Nicole Holton,
  • Agnieszka J. Pietrzyk-Brzezinska,
  • Philipp Hackert,
  • Katherine E. Bohnsack,
  • Markus T. Bohnsack,
  • Markus C. Wahl

DOI
https://doi.org/10.1038/s41467-020-19221-x
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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The DNA helicase ASCC3 is the largest subunit of the activating signal co-integrator complex (ASCC), and its DNA unwinding activity is required for the AlkBH3/ASCC-dependent DNA de-alkylation repair pathway. Here, the authors identify a minimal stable complex of the two ASCC subunits ASCC2 and ASCC3, determine the complex crystal structure and further show that cancer-related mutations at the interface between both proteins reduce ASCC2–ASCC3 affinity.