Exploring the Relationship between Gut Microbiome Composition and Blood Indole-3-acetic Acid in Hemodialysis Patients
Ping-Hsun Wu,
Yu-Fang Tseng,
Wangta Liu,
Yun-Shiuan Chuang,
Chi-Jung Tai,
Chun-Wei Tung,
Kean-Yee Lai,
Mei-Chuan Kuo,
Yi-Wen Chiu,
Shang-Jyh Hwang,
Wei-Chun Hung,
Yi-Ting Lin
Affiliations
Ping-Hsun Wu
Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Yu-Fang Tseng
Department of Family Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Wangta Liu
Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Yun-Shiuan Chuang
Center for Big Data Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Chi-Jung Tai
Department of Family Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
Chun-Wei Tung
Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli 350, Taiwan
Kean-Yee Lai
Post Baccalaureate Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Mei-Chuan Kuo
Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Yi-Wen Chiu
Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Shang-Jyh Hwang
Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Wei-Chun Hung
Department of Microbiology and Immunology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Yi-Ting Lin
Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Indole-3-acetic acid (IAA), a protein-bound uremic toxin resulting from gut microbiota-driven tryptophan metabolism, increases in hemodialysis (HD) patients. IAA may induce endothelial dysfunction, inflammation, and oxidative stress, elevating cardiovascular and cognitive risk in HD patients. However, research on the microbiome–IAA association is limited. This study aimed to explore the gut microbiome’s relationship with plasma IAA levels in 72 chronic HD patients aged over 18 (August 2016–January 2017). IAA levels were measured using tandem mass spectrometry, and gut microbiome analysis utilized 16s rRNA next-generation sequencing. Linear discriminative analysis effect size and random forest analysis distinguished microbial species linked to IAA levels. Patients with higher IAA levels had reduced microbial diversity. Six microbial species significantly associated with IAA levels were identified; Bacteroides clarus, Bacteroides coprocola, Bacteroides massiliensi, and Alisteps shahii were enriched in low-IAA individuals, while Bacteroides thetaiotaomicron and Fusobacterium varium were enriched in high-IAA individuals. This study sheds light on specific gut microbiota species influencing IAA levels, enhancing our understanding of the intricate interactions between the gut microbiota and IAA metabolism.
