Julius-Kühn-Archiv (Feb 2016)

Chlorophyll fluorescence response to herbicide stress in Alopecurus myosuroides

  • Wang, Pei,
  • Li, Hui,
  • Gerhards, Roland

DOI
https://doi.org/10.5073/jka.2016.452.008
Journal volume & issue
no. 452
pp. 57 – 67

Abstract

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Herbicide resistance has been a widely spread problem all over the world. The resistance detection is usually complicated and financial resources consuming. A sensor based monitoring of herbicide resistance would make the resistance detection much faster and spends less. In this research, greenhouse and field experiments with susceptive and resistant populations were conducted to study the chlorophyll fluorescence response of Alopecurus myosuroides after herbicide treatment. The fluorescence sensor WEED-PAM was used for measuring the maximum photosynthesis system II quantum yield (Fv/Fm) of Alopecurus myosuroides after the application for two weeks. The Fv/Fm values of all untreated plants were around 0.7. In the greenhouse, Fv/Fm values of treated susceptive plants reduced significantly from 0.7 to 0.6-0.65 since 3 DAT (days after treatment), but Fv/Fm values of herbicide resistant populations show no significant reductions with ALS and ACCase treatments. With the treatment of PSII inhibitors the Fv/Fm values of susceptive and resistant plants decreased to 0.25 and 0.6 (3DAT) and further to 0.1 and 0.5 (14 DAT), respectively. The field experiments show corresponding results to the greenhouse test. With ALS and ACCase treatment, significant Fv/Fm reduction for the susceptive populations can be detected between 3-7 DAT. During the measurement of PS II inhibitor treatment, Fv/Fm values of susceptive plants decreased continuously from 0.5 to 0.2, while the value of resistant population kept stable in range 0.3-0.4. The prototype WEED-PAM is suitable for the detection of herbicide effects based on Fv/Fm values and takes less time than visual assessment. This research shows the potential usage of fluorescence meter in herbicide resistance detection.

Keywords