Cellular Distribution of NMDA Glutamate Receptor Subunit mRNAs in the Human Cerebellum

Clemens R. Scherzer; G.Bernhard Landwehrmeyer; Julie A. Kerner; David G. Standaert; Zane R. Hollingsworth; Lorrie P. Daggett; Gönül Veliçelebi; John B. Penney, Jr.; Anne B. Young

Neurobiology of Disease (Jan 1997)

Cellular Distribution of NMDA Glutamate Receptor Subunit mRNAs in the Human Cerebellum

Clemens R. Scherzer,
G.Bernhard Landwehrmeyer,
Julie A. Kerner,
David G. Standaert,
Zane R. Hollingsworth,
Lorrie P. Daggett,
Gönül Veliçelebi,
John B. Penney, Jr.,
Anne B. Young

Affiliations

Clemens R. Scherzer: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
G.Bernhard Landwehrmeyer: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
Julie A. Kerner: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
David G. Standaert: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
Zane R. Hollingsworth: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
Lorrie P. Daggett: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
Gönül Veliçelebi: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
John B. Penney, Jr.: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641
Anne B. Young: Department of Neurology, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts, 02114; Department of Neurology, Albert-Ludwigs-University, 79106, Freiburg, Germany; SIBIA Neurosciences, Inc. 505 Coast Boulevard South, La Jolla, California, 92037-4641

Journal volume & issue: Vol. 4, no. 1
pp. 35 – 46

Abstract

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We have used a quantitativein situhybridization method with human ribonucleotide probes to examine the regional and cellular distribution ofN-methyl-d-aspartate receptor (NMDAR) subunit mRNAs in the human cerebellum. Purkinje cells showed very dense labeling for NMDAR1 mRNA, dense labeling for NMDAR2A mRNA, and moderate labeling for NMDAR2D mRNA, whereas labeling for NMDAR2C mRNA was low. Granule cells showed high hybridization signals for the NMDAR1 and NMDAR2C mRNAs and moderate signals for the NMDAR2A and NMDAR2D mRNAs. In addition intense labeling with the NMDAR2B probe was observed in medium-sized neurons with chromophilic cell bodies in the upper part of the granule cell layer, most likely representing Golgi cells. Neurons in the molecular layer, i.e., basket cells and stellate cells, showed moderate hybridization signals for NMDAR1 and NMDAR2D and low signal for NMDAR2C. Each type of cerebellar neuron analyzed displayed a distinct NMDAR2 subunit profile, suggesting that they are likely to have NMDA receptors with distinct properties.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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