STAR Protocols (Dec 2020)

Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

  • Kei Takahashi,
  • Shimpei I. Kubota,
  • Shogo Ehata,
  • Hiroki R. Ueda,
  • Kohei Miyazono

Journal volume & issue
Vol. 1, no. 3
p. 100191

Abstract

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Summary: Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry.For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).

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