Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl<sub>4</sub>-Induced Liver Damage
Amani Taamalli,
Anouar Feriani,
Jesús Lozano-Sanchez,
Lakhdar Ghazouani,
Afoua El Mufti,
Mohamed Salah Allagui,
Antonio Segura-Carretero,
Ridha Mhamdi,
David Arráez-Roman
Affiliations
Amani Taamalli
Department of Chemistry, College of Sciences, University of Hafr Al Batin, P.O. Box 1803, Hafr Al Batin 39524, Saudi Arabia
Anouar Feriani
Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, Gafsa 2112, Tunisia
Jesús Lozano-Sanchez
Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain
Lakhdar Ghazouani
Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, Gafsa 2112, Tunisia
Afoua El Mufti
Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, Gafsa 2112, Tunisia
Mohamed Salah Allagui
Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, Sfax 3018, Tunisia
Antonio Segura-Carretero
Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain
Ridha Mhamdi
Laboratoire de Biotechnologie de l’Olivier, Centre de Biotechnologie de Borj-Cedria, B.P.901, Hammam-Lif 2050, Tunisia
David Arráez-Roman
Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain
Virgin olive oil has demonstrated its effective activity against oxidative stress. However, data on the bioactive effect of olive leaves or their major constituents on the liver are scarce. The present research work was conducted to evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO2) extracts from fresh and dried olive leaves on hepatotoxicity caused by carbon tetrachloride (CCl4) in rat models. For this purpose, healthy albino rats of 180–250 g weight were used. The assessment of biochemical markers was carried out on blood and liver tissue. Then, a histopathological study was carried out on liver tissue. The obtained results showed that fresh and dried olive leaf extracts ameliorate the perturbed biochemical parameters caused by CCl4 treatment. Furthermore, the results registered for the histopathological study are in accordance with the biochemical parameters and the protective capacity of SC-CO2 extracts against DNA damage, indicating that olive leaf extracts helped to improve liver fibrosis caused by CCl4 treatment.
