Archivos Latinoamericanos de Nutrición (Jun 2001)
Avaliação biológica de soja com baixas atividades de inibidores de tripsina e ausência do inibidor Kunitz
Abstract
Cultivar de soja BR 36 com atividades de inibidores de tripsina convencional e linhagem BRM 95-5262, geneticamente modificadas para conter baixas atividades de inibidores de tripsina, contendo 40 e 20 e 30 e 20% de atividades residuais relativa de inibidores de tripsina, respectivamente, foram utilizados para ensaios biológicos com ratos. Os valores médios de PER e NPR indicaram que os tratamentos de soja crua foram inferiores aos tratamentos de soja processada termicamente. Entretanto, para tratamentos de soja processada termicamente não houveram diferenças significativas (p³0,05). Porém, quando as atividades de inibidores de tripsina foram de 8,61 e 8,44 UIT/mg de amostra ou 20 e 30% de atividades residuais relativa das respectivas sojas BR 36 e BRM 95-5262, observou-se que os valores médios de PER e NPR não foram significativos. Os valores médios de CDA e CDV dos tratamentos de sojas cruas foram inferiores à caseína e similares aos tratamentos de sojas processadas termicamente. Portanto, pode-se concluir que a avaliação biológica obtida com a proteína de soja foi dependente das atividades iniciais de inibidores de tripsina e do seu respectivo tratamento térmico e houve vantagem na utilização da soja BRM95-5262 com baixas atividades de inibidores de tripsinaBiological evaluation of soybean line with low trypsin inhibitor activities.The soybean cultivar BR 36 with conventional levels of trypsin inhibitors activity and the soybean line BRM95-5262, which was genetically selected to contain low activity of trypsin inhibitors were used for biological assays with rats. BR 36 and BRM95-5262 contained 40 and 20, and 30 and 20% of relative residual activity of trypsin inhibitors, respectively. The mean values of PER and NPR showed that treatments with crude soybeans were minor than treatments with soybean thermically processed. However, the treatments of thermically processed soybean did not showed significative differences (p³0.05). When the trypsin inhibitors activity were 8.61 and 8.44 UIT/mg of samples or 20 and 30% of relative residual activity of cultivar BR 36 and line BRM95-5262, respectively, it was observed that mean values of PER and NPR were not significatives. The mean values of CDA and CDV of treatments with crude soybeans were minor than treatment with casein and similar to the treatments with soybean thermically processed. So, it can be concluded that the biological evaluation obtained with soybean protein were dependent of initial trypsin inhibitors activities and of its respective thermical treatment. There was advantage in the use of BRM95-5262 soybean line with low trypsin inhibitors activity
