Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)

Hui Lin; Shi-Qiao Ye; Zhao-Hui Xu; Jacqueline S. Penaloza; Mona Aljuhani; Tatyana Vetter; Ming-Tao Zhao; Kim L. McBride

Stem Cell Research (Dec 2022)

Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)

Hui Lin,
Shi-Qiao Ye,
Zhao-Hui Xu,
Jacqueline S. Penaloza,
Mona Aljuhani,
Tatyana Vetter,
Ming-Tao Zhao,
Kim L. McBride

Affiliations

Hui Lin: Center for Cardiovascular Research and The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Shi-Qiao Ye: Center for Cardiovascular Research and The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Zhao-Hui Xu: Center for Vaccines and Immunity, Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA
Jacqueline S. Penaloza: Computational Genomics Group, The Steve and Cindy Rasmussen Institute for Genomic Medicine, Nationwide Children’s Hospital, Columbus, OH, USA
Mona Aljuhani: Center for Cardiovascular Research and The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Tatyana Vetter: Center for Gene Therapy, Nationwide Children’s Hospital, Columbus, OH, USA
Ming-Tao Zhao: Center for Cardiovascular Research and The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA; Corresponding authors at: Center for Cardiovascular Research and The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA.
Kim L. McBride: Center for Cardiovascular Research and The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA; Corresponding authors at: Center for Cardiovascular Research and The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA.

Journal volume & issue: Vol. 65
p. 102958

Abstract

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Epstein-Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) are widely used for banking. This bioresource could be leveraged for creating human iPSC lines to model diseases including CHD. We generated an LCL-derived iPSC line (NCHi001-A) from a patient with congenital aortic valve stenosis. NCHi001-A was EBV and transgenes free, exhibited stem cell-like morphology, expressed pluripotency markers, has a normal karyotype, and could be differentiated into cells of three germ layers in vitro. Relationship inference via a microarray-based analysis showed NCHi001-A is identical to the parental cell line. NCHi001-A can be used for disease modeling, drug discovery, and cell therapy development.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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