Ectopic Expression of <i>AtYUC8</i> Driven by <i>GL2</i> and <i>TT12</i> Promoters Affects the Vegetative Growth of Arabidopsis
Chao Tan,
Jia Song,
Tan Zhang,
Mengxiao Liang,
Suxin Li,
Huabin Liu,
Shuzhen Men
Affiliations
Chao Tan
Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Jia Song
Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Tan Zhang
Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Mengxiao Liang
Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Suxin Li
Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Huabin Liu
College of Life and Health Sciences, Anhui Science and Technology University, Chuzhou 233100, China
Shuzhen Men
Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
Auxin plays an essential role in regulating Arabidopsis growth and development. YUCCA (YUC) family genes encode flavin monooxygenases, which are rate-limiting enzymes in the auxin biosynthetic pathway. Previous studies showed that YUC8 overexpression (YUC8 OE), as well as ectopic expression of YUC8 driven by GL2 (GLABRA 2) and TT12 (TRANSPARENT TESTA 12) promoters, which are specifically expressed in the epidermis and inner seed integument, respectively, produced larger seeds compared to the Col. However, the impact of these transgenic lines on the vegetative growth of Arabidopsis remains unclear. Here, we show that the GL2pro:YUC8-GFP and TT12pro:YUC8-GFP transgenic plants produce a moderate excessive auxin accumulation phenotype compared to the YUC8 OE. These two transgenic lines produced smaller rosette and leaf, higher plant height, fewer branches, and longer siliques. These data will provide a basis for the study of the relationship between the ectopic expression of auxin synthesis genes and crop yield.
