Imaging small molecule-induced endosomal escape of siRNA

Hampus Du Rietz; Hampus Hedlund; Sten Wilhelmson; Pontus Nordenfelt; Anders Wittrup

doi:10.1038/s41467-020-15300-1

Nature Communications (Apr 2020)

Imaging small molecule-induced endosomal escape of siRNA

Hampus Du Rietz,
Hampus Hedlund,
Sten Wilhelmson,
Pontus Nordenfelt,
Anders Wittrup

Affiliations

Hampus Du Rietz: Department of Clinical Sciences, Oncology, Faculty of Medicine, Lund University
Hampus Hedlund: Department of Clinical Sciences, Oncology, Faculty of Medicine, Lund University
Sten Wilhelmson: Department of Clinical Sciences, Oncology, Faculty of Medicine, Lund University
Pontus Nordenfelt: Department of Clinical Sciences, Division of Infection Medicine, Faculty of Medicine, Lund University
Anders Wittrup: Department of Clinical Sciences, Oncology, Faculty of Medicine, Lund University

DOI: https://doi.org/10.1038/s41467-020-15300-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 17

Abstract

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Therapeutic siRNA becomes trapped in endosomes, limiting its efficacy. Here the authors use fluorescently-tagged galectin-9 as a biosensor for membrane damage to monitor endosomal escape of cholesterol-conjugated siRNA following treatment of small molecule membrane-destabilising drugs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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