Electrochemical determination of choline using nortropine-N-oxyl for a non-enzymatic system

Abstract

The electrocatalytical activity of nortropine-N-oxyl (NNO) was evaluated by cyclic voltammetry in pH 7.4 phosphate buffer solution. The anodic peak current for choline was enhanced in a concentration-dependent fashion, showing that choline was oxidized by the electrocatalytic effect of NNO. A high linear response to the choline concentration ranging from 0.1 to 10 mM was obtained. In contrast, 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) could not oxidize choline under the same conditions. Keywords: Choline, Electrocatalytic oxidation, Nitroxyl radical, Non-enzymatic system