Procedures for cryogenic X-ray ptychographic imaging of biological samples

M. Yusuf; F. Zhang; B. Chen; A. Bhartiya; K. Cunnea; U. Wagner; F. Cacho-Nerin; J. Schwenke; I. K. Robinson

doi:10.1107/S2052252516020029

IUCrJ (Mar 2017)

Procedures for cryogenic X-ray ptychographic imaging of biological samples

M. Yusuf,
F. Zhang,
B. Chen,
A. Bhartiya,
K. Cunnea,
U. Wagner,
F. Cacho-Nerin,
J. Schwenke,
I. K. Robinson

Affiliations

M. Yusuf: London Centre for Nanotechnology, University College London, London, England
F. Zhang: London Centre for Nanotechnology, University College London, London, England
B. Chen: London Centre for Nanotechnology, University College London, London, England
A. Bhartiya: London Centre for Nanotechnology, University College London, London, England
K. Cunnea: Research Complex at Harwell, Rutherford Appleton Laboratory, Oxfordshire, England
U. Wagner: Diamond Light Source, Didcot, Oxfordshire, England
F. Cacho-Nerin: Diamond Light Source, Didcot, Oxfordshire, England
J. Schwenke: London Centre for Nanotechnology, University College London, London, England
I. K. Robinson: London Centre for Nanotechnology, University College London, London, England

DOI: https://doi.org/10.1107/S2052252516020029
Journal volume & issue: Vol. 4, no. 2
pp. 147 – 151

Abstract

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Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.

Published in IUCrJ

ISSN: 2052-2525 (Online)
Publisher: International Union of Crystallography
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry: Crystallography
Website: https://journals.iucr.org/m/

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