IUCrJ
(Mar 2017)
Procedures for cryogenic X-ray ptychographic imaging of biological samples
M. Yusuf,
F. Zhang,
B. Chen,
A. Bhartiya,
K. Cunnea,
U. Wagner,
F. Cacho-Nerin,
J. Schwenke,
I. K. Robinson
Affiliations
M. Yusuf
London Centre for Nanotechnology, University College London, London, England
F. Zhang
London Centre for Nanotechnology, University College London, London, England
B. Chen
London Centre for Nanotechnology, University College London, London, England
A. Bhartiya
London Centre for Nanotechnology, University College London, London, England
K. Cunnea
Research Complex at Harwell, Rutherford Appleton Laboratory, Oxfordshire, England
U. Wagner
Diamond Light Source, Didcot, Oxfordshire, England
F. Cacho-Nerin
Diamond Light Source, Didcot, Oxfordshire, England
J. Schwenke
London Centre for Nanotechnology, University College London, London, England
I. K. Robinson
London Centre for Nanotechnology, University College London, London, England
DOI
https://doi.org/10.1107/S2052252516020029
Journal volume & issue
Vol. 4,
no. 2
Abstract
Read online
Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.
Keywords
Published in IUCrJ
ISSN
2052-2525 (Online)
Publisher
International Union of Crystallography
Country of publisher
United Kingdom
LCC subjects
Science: Chemistry: Crystallography
Website
https://journals.iucr.org/m/
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