Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α

Chi Zhang; Lili Xu; Mizuki Endo; Tomoaki Kahyo; Kenji Kikushima; Makoto Horikawa; Makoto Murakami; A.S.M. Waliullah; Md.Mahmudul Hasan; Takumi Sakamoto; Yutaka Takahashi; Shuhei Aramaki; Takeaki Ozawa; Mitsutoshi Setou

Journal of Photochemistry and Photobiology (Dec 2022)

Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α

Chi Zhang,
Lili Xu,
Mizuki Endo,
Tomoaki Kahyo,
Kenji Kikushima,
Makoto Horikawa,
Makoto Murakami,
A.S.M. Waliullah,
Md.Mahmudul Hasan,
Takumi Sakamoto,
Yutaka Takahashi,
Shuhei Aramaki,
Takeaki Ozawa,
Mitsutoshi Setou

Affiliations

Chi Zhang: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Lili Xu: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Mizuki Endo: Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-0033, Japan
Tomoaki Kahyo: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Kenji Kikushima: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Makoto Horikawa: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Hiroshima Research Center for Healthy Aging, Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima, Hiroshima 739-8530, Japan
Makoto Murakami: Graduate School of Medicine, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-8655, Japan
A.S.M. Waliullah: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Md.Mahmudul Hasan: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Takumi Sakamoto: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Yutaka Takahashi: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Shuhei Aramaki: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Department of Radiation Oncology, Hamamatsu University School of Medicine, Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Takeaki Ozawa: Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-0033, Japan
Mitsutoshi Setou: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Corresponding author at: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.

Journal volume & issue: Vol. 12
p. 100150

Abstract

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Light, an inevitable factor for human life, can have negative effects such as cellular inflammation and thus be toxic to cells. Lipids are important biomolecules that are involved in cellular inflammatory responses. Our research revealed the impact of light on cellular lipids. We discovered that blue light irradiation altered cellular lipidome based on non-targeted lipidomic analysis. Inflammation-related lysophospholipids, arachidonic acid, docosahexaenoic acid, and eicosapentaenoic acid, in particular, were significantly increased. We created opto-PiC, an optolipidomic tool based on the optogenetically engineered cPLA2 enzyme. Light-induced lipidomic changes were reversed by opto-PiC. Furthermore, the results showed that opto-PiC reduced cell death rate under blue light. Overall, our research provided lipidomic insights and optolipidomics approach into light toxicity. This could help the therapy development in the future for inflammation-related disease.

Published in Journal of Photochemistry and Photobiology

ISSN: 2666-4690 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: https://www.journals.elsevier.com/journal-of-photochemistry-and-photobiology

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