Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk (Feb 2018)
Effect of Four Weeks Exercise Prior Preparation before Alzheimer's Induction on the Levels of Nerve Growth Factor and Beta Amyloid in the Hippocampus of Wistar Male Rats
Abstract
Abstract Background: The aim of this study was to investigate the effect of four weeks exercise prior preparation before Alzheimer's induction on the levels of nerve growth factor and beta amyloid in the hippocampus of Wistar male rats Materials and Methods: 84 male Wistar rats (8 weeks old) with a weighing average of 20 ± 195 grams from Pasteur Institute of Iran were prepared, and rats were randomly divided to two exercise (4 weeks aerobic training on a treadmill with a gradient of 0 degrees, 5 days a week for 4 weeks) and rest groups. Then, the rats of each group were randomly assigned to 3 sub groups of 14 numbers, injection Aβ1-42, control, and non-injected. 48 hours after the last exercise session, injections into the hippocampus amyloid beta or Dimethyl sulfoxide were performed. Seven days after surgery, the rats of each group were randomly sacrificed or subjected to behavioral testing. To determine the levels of of nerve growth factor and beta amyloid Sampling was performed from the hippocampus and plasma of animals, a Morris water maze test was used for spatial memory test. Kolmogorov-Smirnov test (KS) and analysis of variance were used to analyze the data. Results: The results of one-way analysis of variance showed that there was a significant difference between the levels of amylohyd of hippocampus and NGF in different groups. Also, the results of the probe test for spatial memory showed that the time spent on the target circle in the Aβ1-42 injection group was significantly lower than the other groups (p≤ 0.01). Also, the exercise and exercise + sham groups had a significantly better performance than control group. Conclusion: It seems that performing physical activity before induction of Alzheimer's in rats is a kind of countermeasures and preeclampsia with physiological disorders and progression of the disease.
