Study of anatomical feature of in vitro and ex vitro regeneration plant of Sophora Alopecuroides l.

Abstract

The success of shoot and rooting from the nodal shoot segments of Sophora alopecuroides L. were induced in vitro condition. However, transferring and acclimatizing the plantlets to under soil or ex vitro condition were difficult. This study investigated plant survival with anatomical changes in plantlets while transferring from in vitro to ex vitro conditions to investigate vascular cylinder variations. The ex vitro rooting of the in vitro regenerated shoots, after having been treated with 500 mg/L IBA, showed a success rate of 80 per cent. These plantlets were rooted and acclimatized simultaneously in ex vitro condition.

Keywords

• Sophora alopecuroides
• regeneration
• ex vitro rooting
• anatomy
• central cylinder