Development of a High Yielded Chlorsulfuron-Resistant Soybean (<i>Glycine max</i> L.) Variety through Mutation Breeding

Abstract

This study was conducted to develop a novel herbicide resistance soybean using ethyl methanesulfonate (EMS) mutagen. In this study, 0.1% of EMS mutagen was applied to the soybean [Glycine max (L.) cv Arısoy] seeds. A single resistant mutant was selected in the M2 population evaluated under field and greenhouse conditions. The AHAS gene regions of the herbicide-resistant mutant progeny were mapped, and the nucleotide changes were defined conferring herbicide resistance. The sequence analysis of the AHAS gene indicated that three nucleotide substitutions were detected such as 407 (C/T), 532 (C/T), and 1790 (C/T). According to the AHAS gene protein sequence of Arabidopsis thaliana, Ala155Val, Pro197Ser, and Thr616Met amino acid alterations were found in the progeny of the resistant mutant. Pro197Ser alteration was common in all the progeny, while the others were diverse. The wild-type and the mutant plants were compared for seed yield, number of pods per plant, stem height to the first pod, 1000-seed weight, and physiological maturity days for two subsequent years. No statistical difference was found between the mutant and wild types with respect to seed yield and its components. The agronomic data indicated that EMS provided target-site resistance to sulfonylureas (SU) with no tradeoff between yield components and resistance.

Keywords

• AHAS gene
• EMS mutagenesis
• herbicide resistance
• chlorsulfuron
• <i>Glycine max</i>