Genome-Wide Identification of Cotton (<i>Gossypium</i> spp.) Trehalose-6-Phosphate Phosphatase (TPP) Gene Family Members and the Role of <i>GhTPP22</i> in the Response to Drought Stress
Weipeng Wang,
Hua Cui,
Xiangfen Xiao,
Bingjie Wu,
Jialiang Sun,
Yaxin Zhang,
Qiuyue Yang,
Yuping Zhao,
Guoxiang Liu,
Tengfei Qin
Affiliations
Weipeng Wang
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266100, China
Hua Cui
Key Laboratory of Cell and Gene Circuit Design, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Xiangfen Xiao
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China
Bingjie Wu
College of Agriculture, Liaocheng University, Liaocheng 252059, China
Jialiang Sun
College of Agriculture, Liaocheng University, Liaocheng 252059, China
Yaxin Zhang
College of Agriculture, Liaocheng University, Liaocheng 252059, China
Qiuyue Yang
College of Agriculture, Liaocheng University, Liaocheng 252059, China
Yuping Zhao
College of Agriculture, Liaocheng University, Liaocheng 252059, China
Guoxiang Liu
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266100, China
Tengfei Qin
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266100, China
Trehalose-6-phosphate phosphatase (TPP) is a key enzyme involved in trehalose synthesis in higher plants. Previous studies have shown that TPP family genes increase yields without affecting plant growth under drought conditions, but their functions in cotton have not been reported. In this study, 17, 12, 26 and 24 TPP family genes were identified in Gossypium arboreum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum, respectively. The 79 TPP family genes were divided into three subgroups by phylogenetic analysis. Virus-induced gene silencing (VIGS) of GhTPP22 produced TRV::GhTPP22 plants that were more sensitive to drought stress than the control plants, and the relative expression of GhTPP22 was decreased, as shown by qRT–PCR. Moreover, we analysed the gene structure, targeted small RNAs, and gene expression patterns of TPP family members and the physicochemical properties of their encoded proteins. Overall, members of the TPP gene family in cotton were systematically identified, and the function of GhTPP22 under drought stress conditions was preliminarily verified. These findings provide new information for improving drought resistance for cotton breeding in the future.
