Bioingeniøren (Oct 2012)
Flow cytometry in clinical practise
Abstract
In the past 20 years flow cytometry has developed from being a research-tool to becoming an important part of the diagnostic arsenal of haematological and immunological diseases. Increased knowledge regarding normal hematopoietic differentiation and cells in peripheral blood has made identification of abnormal populations possible. The rapid evolution of modern flow cytometers, as well as new monoclonal antibodies and fluorochromes, has increased the sensitivity of the method, creating new applications. Flow cytometry has an advantage over other molecular methods, in that it is relatively rapid. A broader description of normal, as well as pathological cells, may also be given. However, flow cytometry is a specialised analysis, and extensive technical and analytical competence is required. The analysis is relatively costly, and should therefore only be used where there is a sound clinical indication. This review will provide a short introduction to the technology, elucidating the potential and the limitations of this method. Some examples of how flow cytometry is used in clinical practise at St. Olavs Hospital will be given, as well as indicators of possible future developments.
