Genotyping-by-sequencing application on diploid rose and a resulting high-density SNP-based consensus map

Abstract

Genetics: A DNA map of roses A genetic map of rose DNA could help flower breeders develop ornamental crops that are more resistant to disease or have other desirable traits. Rose breeder, David Byrne and molecular geneticist, Patricia Klein along with their colleagues from Texas A&M University in College Station, USA, bred five different strains of ‘diploid’ rose, each with two full sets of chromosomes, to create 234 offspring plants. Using the full genome from strawberry, a closely related species, as a reference, the researchers then looked for sites in the genome where either single DNA letters differed between individual offspring or where short sequences of DNA repeated themselves to create easily identifiable genetic markers. They created genetic maps of each parental cross, and then formed a consensus map that can now serve as a tool for future genetically guided breeding efforts of horticulturally important traits.