Frontiers in Microbiology (Oct 2021)

The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1

  • Jianwei Zhou,
  • Jianwei Zhou,
  • Yonghui Qiu,
  • Yonghui Qiu,
  • Ning Zhu,
  • Ning Zhu,
  • Linyi Zhou,
  • Linyi Zhou,
  • Beining Dai,
  • Beining Dai,
  • Xufei Feng,
  • Xufei Feng,
  • Lei Hou,
  • Lei Hou,
  • Jue Liu,
  • Jue Liu

DOI
https://doi.org/10.3389/fmicb.2021.751382
Journal volume & issue
Vol. 12

Abstract

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Porcine circovirus type 4 (PCV4) is an emerging etiological agent which was first detected in 2019. The nucleolar localization signal (NoLS) of PCV4 Cap protein and its binding host cellular proteins are still not elucidated. In the present study, we discovered a distinct novel NoLS of PCV4 Cap, which bound to the nucleolar phosphoprotein nucleophosmin-1 (NPM1). The NoLS of PCV4 Cap and serine-48 residue at the N-terminal oligomerization domain of NPM1 were necessary for PCV4 Cap/NPM1 interaction. Furthermore, the charge property of serine residue at position 48 of the NPM1 was crucial for its oligomerization and interaction with PCV4 Cap. In summary, our findings show for the first time that the PCV4 Cap NoLS and the NPM1 oligomerization determine the interaction of Cap/NPM1.

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