Influence of complexes of antisense-oligonucleotides with polymeric carriers on content of cellular prion in rat organs

Abstract

Сonjugates of antisense ODN (asODN) with three newly synthesized samples (MP-2, MP-2, MP-27) of polymer carriers based on DMAEM have been studied. It was found that these best conjugates could be created by mixing 0.5 % solution of polymers with asODN. It was found that a survival time of sperm cells was 96 h under action of 0.5 % solution of polymers MP-2 and MP-3. The survival time of sperm cells was increseaced to 120 h at treatment of cells with 0.5% solution of MP-27 polymer. That had lower cytotoxicity comparing to MP-2 and MP-3 polymers. The effectiveness of transporting system of these conjugates and its influence on cellular prion protein were studied. The results of Western-blot analysis showed that the injection of asODN conjugates with DMAEM into rats caused a decrease in PrPC in spleen, intestines and brain. AsODN conjugates injected with MP-27 carriers demonstrated that the content of cellular prion in spleen was decreased by 9 % after 2 days and by 32 % after 7 days. The contents of cellular prion in the intestine decreased by 38 % after 2 days and by 55 % after 7 days. The content of cellular prion in brain tissues decreased by 28 % after 2 days and by 34 % after 7 days, respectively. Thus, complexes of asODN with DMAEM descrease the content of cellular prion in rat organs and tissues.

Keywords

• cellular prion
• brain
• intestines
• spleen
• oligonucleotides
• DMAEM conjugates