Effects of Temperature on Growth, Molting, Feed Intake, and Energy Metabolism of Individually Cultured Juvenile Mud Crab <i>Scylla paramamosain</i> in the Recirculating Aquaculture System
Jiahao Liu,
Ce Shi,
Yangfang Ye,
Zhen Ma,
Changkao Mu,
Zhiming Ren,
Qingyang Wu,
Chunlin Wang
Affiliations
Jiahao Liu
Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
Ce Shi
Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
Yangfang Ye
Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
Zhen Ma
Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education, 52 Heishijiao Street, Dalian 116023, China
Changkao Mu
Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
Zhiming Ren
Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
Qingyang Wu
Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
Chunlin Wang
Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
An eight-week experiment was conducted to investigate the effects of temperature (20, 25, 30, and 35 °C) on growth performance, feed intake, energy metabolism, antioxidant capacity, and the stress response of juvenile Scylla paramamosain in a recirculating aquaculture system. The results showed that the survival rate of the 35 °C group was 80.36 ± 5.92%, significantly lower than that of the other three groups (100%). The high molt frequency of mud crabs was observed in high-temperature groups, accompanied by a higher ecdysone level and ecdysone receptor gene expression but lower molt inhibitory hormone gene expression. However, the molt increment (73.58 ± 2.18%), food intake, and feed conversion efficiency showed a parabolic trend, with the lowest value found in the 35 °C group. Oxygen consumption rate and ammonia excretion rate increased with the increasing temperature, and oxygen-nitrogen ratio, lactic acid, triglyceride, total cholesterol, glucose, and cortisol peaked at 35 °C. Temperature also significantly affected the antioxidant system of S. paramamosain. Crabs in the 25 °C and 30 °C had a significantly higher total antioxidant capacity and lower malondialdehyde compared with the 35 °C group (p S. paramamosain was 28.5–29.7 °C.
