Molecules (Apr 2022)

Synthesis of Deuterium-Labeled Vitamin D Metabolites as Internal Standards for LC-MS Analysis

  • Akiko Nagata,
  • Kazuto Iijima,
  • Ryota Sakamoto,
  • Yuka Mizumoto,
  • Miho Iwaki,
  • Masaki Takiwaki,
  • Yoshikuni Kikutani,
  • Seketsu Fukuzawa,
  • Minami Odagi,
  • Masayuki Tera,
  • Kazuo Nagasawa

DOI
https://doi.org/10.3390/molecules27082427
Journal volume & issue
Vol. 27, no. 8
p. 2427

Abstract

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Blood levels of the vitamin D3 (D3) metabolites 25-hydroxyvitamin D3 (25(OH)D3), 24R,25-dihydroxyvitamin D3, and 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) are recognized indicators for the diagnosis of bone metabolism-related diseases, D3 deficiency-related diseases, and hypercalcemia, and are generally measured by liquid-chromatography tandem mass spectrometry (LC-MS/MS) using an isotope dilution method. However, other D3 metabolites, such as 20-hydroxyvitamin D3 and lactone D3, also show interesting biological activities and stable isotope-labeled derivatives are required for LC-MS/MS analysis of their concentrations in serum. Here, we describe a versatile synthesis of deuterium-labeled D3 metabolites using A-ring synthons containing three deuterium atoms. Deuterium-labeled 25(OH)D3 (2), 25(OH)D3-23,26-lactone (6), and 1,25(OH)2D3-23,26-lactone (7) were synthesized, and successfully applied as internal standards for the measurement of these compounds in pooled human serum. This is the first quantification of 1,25(OH)2D3-23,26-lactone (7) in human serum.

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