Effect of phosphorus and sodium acetate on lipid accumulation from Ankistrodesmus sp. IFRPD 1061 in an open pond
Paninee Jarungkeerativimol,
Afrasiab Khan Tareen,
Imrana Niaz Sultan,
Muhammad Waseem Khan,
Pramuk Parakulsuksatid
Affiliations
Paninee Jarungkeerativimol
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok, 10900, Thailand
Afrasiab Khan Tareen
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok, 10900, Thailand; Department of Biotechnology, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, 87300, Balochistan, Pakistan; Corresponding author. Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok, 10900, Thailand.
Imrana Niaz Sultan
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok, 10900, Thailand; Department of Biotechnology, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, 87300, Balochistan, Pakistan
Muhammad Waseem Khan
Department of Biotechnology, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, 87300, Balochistan, Pakistan
Pramuk Parakulsuksatid
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok, 10900, Thailand; Corresponding author.
Ankistrodesmus sp, has been comprehensively studied for their potential in the production of biodiesel due to their biomass productivity and high lipid content. This study examined the biomass productivity, and concentration, lipid productivity, and concentration, and lipid contents of Ankistrodesmus sp. IFRPD 1061 under several phosphorus concentrations. The optimum conditions were attained at 0.12 g/L KH2PO4. The highest lipid content reached to 35.950 ± 4.253% (w/w) in 22 days cultivation. An open pond cultivation system was used with the addition of 10 mM sodium acetate on every fourth day (0, 4, 8 and 12) of cultivation and KH2PO4 on twelfth day of cultivation. The obtained biomass productivity and concentration, lipid productivity and concentration and lipid content were 0.709 ± 0.027 g/L, 48.304 ± 1.894 mg/L/day, 0.214 ± 0.004 g/L 14.550 ± 0.215 mg/L/day and 30.154 ± 1.627% (w/w) in 14 days of cultivation, respectively. The results exhibited that addition of 10 mM sodium acetate and KH2PO4 may enhance lipid accumulation within algae cells in an open pond cultivation system.
