Química Nova (Jan 2012)

Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

  • Yara P. Cid,
  • Thais P. Ferreira,
  • Deborah M. V. C. Medeiros,
  • Rodrigo M. Oliveira,
  • Nathália C. C. Silva,
  • Viviane S. Magalhães,
  • Fábio B. Scott

DOI
https://doi.org/10.1590/S0100-40422012001000029
Journal volume & issue
Vol. 35, no. 10
pp. 2063 – 2066

Abstract

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A fast and efficient method has been developed and validated for the determination of fipronil in bovine plasma. Samples were subjected to solid-phase extraction (SPE) followed by reversed phase liquid chromatography (LC) separation, using acetonitrile/water (60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet (UV) detection at 210 nm. Ethiprole was used as the internal standard (IS). The method was found to be linear over the range 5-500 ng/mL (r = 0.999). The limit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfully applied to monitor plasma concentrations following subcutaneous administration of fipronil in cattle.

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