Nature Communications (Nov 2018)

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

  • Wenhua Zhou,
  • Li Hu,
  • Liming Ying,
  • Zhen Zhao,
  • Paul K. Chu,
  • Xue-Feng Yu

DOI
https://doi.org/10.1038/s41467-018-07324-5
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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Isothermal DNA amplification techniques are useful for diagnostic applications in place of traditional PCR. Here the authors describe CRISDA, which combines CRISPR–Cas9 with strand displacement amplification and exhibits attomolar sensitivity and single-nucleotide specificity in DNA detection.