Evaluation of Intranasal Delivery of Human Endometrial Stem Cells to the Substantia Nigra and Their Therapeutic Effects on Rotational Behavior Recovery in Mice Model of Parkinson's Disease

Abstract

Background and Aim: Recent findings in cell therapy have presented new perspectives and opportunities for the treatment of neurodegenerative disease. The experimental research with intranasal (IN) administration of Stem Cells in Parkinson’s disease (PD) mouse model can work and in some cases induce major, long-lasting improvement. Adult Human endometrial derived stem cells (HEDSCs), a readily obtainable type of mesenchymal stem-like cell were used to generate dopaminergic cells and for cell therapy. In this study, we investigated the therapeutic effects of IN-delivered HEDSCs in mice model of Parkinson. Materials and Methods: In this experimental research, 35 male mouse weighting 25-30 g were divided into 5 groups. On day 120 post cell administration, the rotational behavior was measured. Immunohistochemistry staining was used to detect HEDSCs in mice brain. Findings: IN application of HEDSCs resulted in the appearance of cells in the substantia nigra (SN) and decrease in the rotational behavior of case group. Conclusion: HEDSCs are a highly inducible source of allogenic stem cells that improve Parkinson’s disease.

Keywords

• Endometrial stem cells
• Intranasal delivery
• Mice
• Parkinson’s disease