Comparative chromosome G-banding analysis of long-tailed macaque (Macaca fascicularis) and relationship to human (Homo sapiens)

Abstract

This research is the first report of the comparative chromosome between long-tailed macaque (Macaca fascicularis) and human (Homo sapiens) using G-banding. Blood samples from four male and three female macaques were used. Their chromosomes were prepared using lymphocyte cultures at 37oC, 72 hours and detected using G-banding. The results showed the diploid chromosome number of 42, 18 metacentric and 22 submetacentric chromosomes. The satellite chromosome was on the short arm of chromosome 13. The X chromosome was a medium submetacentric and the Y was the smallest telocentric chromosome. By using G-banding, the macaque chromosome 5, 12, 13, 19 and X are identical to those of humans. The short arm and long arm of chromosome 13 of macaque were similar to chromosome 22 and 15 of human respectively. We indicate that macaque chromosome was split to two human chromosomes. The macaque and human chromosomes 1, 3, 6-11, 14, 17 and 20 were relatively similar. The macaque and human chromosome 1 is a pericentric inversion chromosome, indicating that the alternative construction of the chromosome cooperates with the centomere. There were 6 macaque chromosomes which were from different human, 2, 4, 15, 16, 18 and Y. All results demonstrate that the long-tailed macaque and human have are evolutionary relationship.

Keywords

• G-banding
• long-tailed macaque (Macaca fascicularis)
• human (Homo sapiens)