Rapid detection protocol ofFusarium oxysporum f. sp.cubense race 1 inbanana

Abstract

Fusarium wilt of banana is a typical vascular wilt disease caused byFusarium oxysporum f.sp.cubense. The genetic improvement program of the crop needs tools that allow the quickdetection of fungal phytopathogen. The objective of this work was to establishment a simpledetection protocol ofF. oxysporum race 1 inMusa spp. samples by polymerase chain reaction(PCR). The principal related aspects in this protocol are the sampling, fungalin vitro culture,quick DNA extraction method, PCR protocol, electrophoresis separation of PCR products, andgeneral considerations during the work.

Keywords

• fusarium wilt of banana
• pcr
• dna extraction
• musa spp.