L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast

Lena Hochrein; Leslie A. Mitchell; Karina Schulz; Katrin Messerschmidt; Bernd Mueller-Roeber

doi:10.1038/s41467-017-02208-6

Nature Communications (May 2018)

L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast

Lena Hochrein,
Leslie A. Mitchell,
Karina Schulz,
Katrin Messerschmidt,
Bernd Mueller-Roeber

Affiliations

Lena Hochrein: Cell2Fab Research Unit, University of Potsdam
Leslie A. Mitchell: Institute for Systems Genetics, New York University Langone School of Medicine
Karina Schulz: Cell2Fab Research Unit, University of Potsdam
Katrin Messerschmidt: Cell2Fab Research Unit, University of Potsdam
Bernd Mueller-Roeber: Department of Molecular Biology, University of Potsdam

DOI: https://doi.org/10.1038/s41467-017-02208-6
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

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The International Synthetic Yeast Sc2.0 project has built Cre recombinase sites into synthetic chromosomes, enabling rapid genome evolution. Here the authors demonstrate L-SCRaMbLE, a light-controlled recombinase tool with improved control over recombination events.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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