A mini-review on diagnosis of contagious caprine pleuropneumonia

Abstract

Diagnosis of contagious caprine pleuropneumonia is imperative for timely detection and devising interventions that prevent disease spread and loss to farmers. Diagnosis of contagious caprine pleuropneumonia involves clinical signs, gross morphological lesions on postmortem, histopathology, culture and isolation, hematological, biochemical, serological and molecular diagnostic tests. Culture and isolation confirms the disease however it has been costly, cumbersome and difficult owing to the requirements of specific media, slow and difficult growth of causative agent Mycoplasma capricolum subsp. capripneumoniae. With the recent developments, diagnosis has comparatively eased by novel readymade media, advanced serological latex agglutination test (LAT), competitive enzyme linked immunosorbent assay (cELISA) or gene-based amplification of DNA, viz. polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), hybridization and sequencing than the cumbersome and lengthy conventional tests; however they have financial implications and require sophisticated laboratory infrastructure and technical manpower. The latex agglutination test (LAT) is rapid, simple, and better test for field and real-time diagnosis applicable to whole blood or serum and is more sensitive than the compliment fixation test (CFT) and easier than the cELISA. PCR and monoclonal antibody based ELISA being specific aid to confirmation of CCPP. Future thrust is on developing rapid, sensitive, and specific tests that are cheap and convenient for field application.

Keywords

• Contagious caprine pleuropneumonia
• Culture
• Diagnosis
• Enzyme linked immunosorbent assay
• Mycoplasma
• Polymerase chain reaction