Core PCP mutations affect short-time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing
Romina Piscitello-Gómez,
Franz S Gruber,
Abhijeet Krishna,
Charlie Duclut,
Carl D Modes,
Marko Popović,
Frank Jülicher,
Natalie A Dye,
Suzanne Eaton
Affiliations
Romina Piscitello-Gómez
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; National Phenotypic Screening Centre, University of Dundee, Dundee, United Kingdom
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany; Center for Systems Biology Dresden, Dresden, Germany
Laboratoire Physico-Chimie Curie, CNRS UMR 168, Institut Curie, Université PSL, Sorbonne Université, Paris, France; Max Planck Institute for Physics of Complex Systems, Dresden, Germany; Université Paris Cité, Laboratoire Matière et Systèmes Complexes, Paris, France
Carl D Modes
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany; Center for Systems Biology Dresden, Dresden, Germany
Marko Popović
DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany; Center for Systems Biology Dresden, Dresden, Germany; Max Planck Institute for Physics of Complex Systems, Dresden, Germany
DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany; Center for Systems Biology Dresden, Dresden, Germany; Max Planck Institute for Physics of Complex Systems, Dresden, Germany
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany; Mildred Scheel Nachwuchszentrum P2, Medical Faculty, Technische Universität Dresden, Dresden, Germany
Suzanne Eaton
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany; Center for Systems Biology Dresden, Dresden, Germany; Biotechnologisches Zentrum, Technische Universität Dresden, Dresden, Germany
How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing of Drosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. Previously, we showed that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned (Etournay et al., 2015). Here, we show that these active cellular behaviors are not guided by the core planar cell polarity (PCP) pathway, a conserved signaling system that guides tissue development in many other contexts. We find no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation they do not significantly affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes during Drosophila pupal wing morphogenesis do not require core PCP as an orientational guiding cue.
