The impact of different feeds on DNA methylation, glycolysis/gluconeogenesis signaling pathway, and gene expression of sheep muscle

Feng Song; Zaccheaus Pazamilala Akonyani; Ying Li; Deqiqige Su; Lantuya Wu; Yue Pang; Sile Hu; Dubala Wu; Chun Li; Ding Yang; Jianghong Wu

doi:10.7717/peerj.13455

PeerJ (May 2022)

The impact of different feeds on DNA methylation, glycolysis/gluconeogenesis signaling pathway, and gene expression of sheep muscle

Feng Song,
Zaccheaus Pazamilala Akonyani,
Ying Li,
Deqiqige Su,
Lantuya Wu,
Yue Pang,
Sile Hu,
Dubala Wu,
Chun Li,
Ding Yang,
Jianghong Wu

Affiliations

Feng Song: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Zaccheaus Pazamilala Akonyani: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Ying Li: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Deqiqige Su: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Lantuya Wu: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Yue Pang: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Sile Hu: College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Dubala Wu: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Chun Li: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China
Ding Yang: Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia, China
Jianghong Wu: College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China

DOI: https://doi.org/10.7717/peerj.13455
Journal volume & issue: Vol. 10
p. e13455

Abstract

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DNA methylation is an important epigenetic regulatory form that regulates gene expression and tissue development. This study compared the effects of high fiber, low protein (HFLP) and low fiber, high protein (LFHP) diets on the DNA methylation profile of twin lambs’ muscles, their effect on glycolysis/gluconeogenesis and related pathways by transcriptome and deep whole-genome bisulfite sequencing (WGBS). Results identified 1,945 differentially methylated regions (DMRs) and 1,471 differentially methylated genes (DMGs). Also, 487 differentially expressed transcripts belonging to 368 differentially expressed genes (DEGs) were discovered between the twin lambs under different diets. Eleven overlapped genes were detected between the DEGs and the DMGs. FKBP5 and FOXO1 were detected to be significantly different. The FOXO1 regulated cAMP and the glycolysis/gluconeogenesis pathways. The glycolysis/gluconeogenesis, and the FOXO pathways were significantly enriched. The expressions of HOMER1 and FOXO1 in the HFLP group were significantly higher than those in the LFHP group. There is a significant correlation between the upregulated gene expression and hypomethylation of HOMER1 and FOXO1 gene in HFLP group. The results showed that FOXO1 induces PDK4 expression in muscle while regulating FKBP5 activity, which stimulates glucose production by activating specific gluconeogenesis target genes. The FOXO1 was able to regulate the glucose metabolism, the cAMP and the occurrence of glycolysis/gluconeogenesis pathways. This study showed that feed type can affect the methylation levels of the glycolysis related gluconeogenesis genes and interaction pathways, providing new ideas for a better understanding of the regulation of muscle energy metabolism and feed development.

Published in PeerJ

ISSN: 2167-8359 (Online)
Publisher: PeerJ Inc.
Country of publisher: United States
LCC subjects: Medicine; Science: Biology (General)
Website: https://peerj.com/

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