Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Yingxin Ma; Mingxiu Wang; Wei Li; Zhiping Zhang; Xiaowei Zhang; Tianwei Tan; Xian-En Zhang; Zongqiang Cui

doi:10.1038/ncomms15318

Nature Communications (May 2017)

Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Yingxin Ma,
Mingxiu Wang,
Wei Li,
Zhiping Zhang,
Xiaowei Zhang,
Tianwei Tan,
Xian-En Zhang,
Zongqiang Cui

Affiliations

Yingxin Ma: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
Mingxiu Wang: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
Wei Li: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
Zhiping Zhang: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
Xiaowei Zhang: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
Tianwei Tan: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology
Xian-En Zhang: CAS Center for Biological Macromolecules, National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Zongqiang Cui: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/ncomms15318
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 8

Abstract

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Visualizing single genomic loci in living cells remains technically challenging. Here the authors adopt a strategy of labelling a pair of transcription activator-like effectors (TALEs) with differently coloured quantum dots and use them to identify integrated HIV-1 proviral DNA sequences in living cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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