A Novel Strategy for Unveiling Spatial Distribution Pattern of Gallotannins in <i>Paeonia rockii</i> and <i>Paeonia ostii</i> Based on LC–QTRAP–MS
Zhangzhen Bai,
Rui Yu,
Tiantian Zheng,
Daoyang Sun,
Yang Zhou,
Junman Tang,
Huili Zhu,
Guangning Li,
Lixin Niu,
Lu Cui,
Rui Du,
Jing Zhang,
Yanlong Zhang
Affiliations
Zhangzhen Bai
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
Rui Yu
College of Horticulture, China Agricultural University, Beijing 100193, China
Tiantian Zheng
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
Daoyang Sun
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
Yang Zhou
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
Junman Tang
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
Huili Zhu
College of Horticulture, Northwest A&F University, Yangling 712100, China
Guangning Li
SCIEX China, Beijing 100015, China
Lixin Niu
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
Lu Cui
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
Rui Du
College of Innovation and experiment, Northwest A&F University, Yangling 712100, China
Jing Zhang
College of Horticulture, Northwest A&F University, Yangling 712100, China
Yanlong Zhang
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
Gallotannins (GTs) are a series of hydrolyzable tannins with multiple health-promoting effects. In this study, an integrated liquid chromatography tandem mass spectrometry (LC–MS/MS) strategy was developed for unveiling the spatial distribution pattern of GTs in the emerging oilseed crops Paeonia rockii and P. ostii. According to the fragmentation behavior of the representative GT (1,2,3,4,6-penta-O-galloyl-β-D-glucose, PGG), the diagnostic neutral loss (NL) of 170 Da was chosen for the non-targeted screening of GT precursors. Simultaneously, the tandem mass spectrometry spectrum (MS/MS) information was acquired through an enhanced product ion (EPI) scan. Nine major GTs were identified in tree peony. To quantify the targeted GTs in different tissues of tree peony, we established a multiple reaction monitoring (MRM)–enhanced product ion (EPI)-based pseudo-targeted approach under the information-dependent acquisition (IDA) mode. The quantitative results show that the GT compounds were ubiquitous in tree peony plants with diverse structures. The typical GT PGG was mainly distributed in roots, leaves, and petals. This strategy can also be utilized for metabolite characterization and quantification in other substrates.
