Zhongguo youzhi (Nov 2022)

响应面法优化生物酶法催化脱臭馏出物甲酯化Optimization of bioenzyme-catalyzed methyl esterification of deodorized distillates by response surface methodology

  • 武文华1, 关国华1, 蒋一鸣1, 宋莲芳2, 王宽2, 耿鑫琪2, 邓军2，徐伟2, 徐逍然1, 童强1WU Wenhua1, GUAN Guohua1, JIANG Yiming1, SONG Lianfang2, WANG Kuan2, GENG Xinqi2, DENG Jun2, XU Wei2, XU Xiaoran1, TONG Qiang1

DOI
https://doi.org/10.19902/j.cnki.zgyz.1003-7969.220401
Journal volume & issue
Vol. 47, no. 11
pp. 143 – 146

Abstract

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为实现脱臭馏出物绿色、清洁、高效甲酯化，达到环保生产的目的，通过单因素实验和响应面实验对生物酶法催化脱臭馏出物甲酯化反应的工艺条件进行了优化，测定了反应前后维生素 E含量的变化，并将新工艺应用于实际生产线。结果表明：生物酶法催化脱臭馏出物甲酯化的最佳反应条件为加水量1%(以脱臭馏出物质量计)、甲醇用量11.5%(以脱臭馏出物质量计)、固定化脂肪酶用量0.3%(以脱臭馏出物质量计)、反应温度35.6 ℃，在最佳条件下反应8 h脱臭馏出物中游离脂肪酸酯化率达到92.59%，甘油酯酯化率达到94.95%；生物酶法催化脱臭馏出物甲酯化反应过程中维生素 E损失少，收率达到93%；与浓硫酸催化甲酯化工艺相比，产品维生素E(50%维生素E)总收率提高了3.47百分点，植物甾醇总收率提高了3.26百分点。综上，生物酶法催化脱臭馏出物甲酯化，可以得到较高的酯化率，提高了产品维生素 E和植物甾醇收率。 In order to realize the green, clean and efficient methyl esterification of deodorized distillates and achieve the goal of environmental protection, the reaction conditions of the bioenzyme-catalyzed methyl esterification of deodorized distillates were optimized by single-factor experiment and response surface methodology, and the changes of vitamin E content before and after the reaction were measured. In addition, the new process was applied to the actual production line. The results showed that the optimal reaction conditions for the methyl esterification of deodorized distillates catalyzed by bioenzyme were as follows: water dosage 1%(based on the mass of deodorized distillates), methanol dosage 11.5% (based on the mass of deodorized distillates), immobilized lipase dosage 0.3% (based on the mass of deodorized distillates), reaction temperature 35.6 ℃. Reaction under the optimal conditions for 8 h, the esterification rate of free fatty acid and glycerol ester in the deodorized distillates reached 92.59% and 94.95%, respectively. In the methyl esterification of the bioenzyme-catalyzed deodorized distillates, the loss of vitamin E was low, and its yield reachedg 93%. The total yield of vitamin E (50% vitamin E) increased by 3.47 percentage points and the total yield of phytosterols increased by 3.26 percentage points compared with the concentrated sulfuric acid catalyzed methyl esterification process. In conclusion, the bioenzyme-catalyzed methyl esterification of deodorized distillates posses higher esterification rates and yields of vitamin E and phytosterols.

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