EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS

Abstract

Goal of the study: to investigate the impact of isoniazid combined with silver nanoparticles on the isoniazid resistant strains of tuberculous mycobacteria and the course of experimental of tuberculosis caused by the above strains.Materials and methods. The electrochemical metal resolution was used to obtain silver nanoparticles, the average size of nanoparticles made 3-60 nm. The investigated concentrations of silver nanoparticles made 5; 25; 50 µg/ml. The isoniazid was used only in one concentration of 1 mg/ml. Totally there were 651 in vitro tests.The experimental tuberculosis model included infecting mice (totally 68) with two-week virulent multiple drug resistant culture of M. tuberculosis.Results. In vitro tests proved that isoniazid combined with silver nanoparticles fully or significantly suppressed the growth of MDR TB strain in 49.2% of cases. The minimal inhibitory concentration of nanoparticles in the combination with isoniazid made 2.5 µg/ml and minimal bactericidal concentration made 5 µg/ml. Atomic force microscopy detected the changes in morphometric parameters of MDR tuberculous mycobacteria after exposure to silver nanoparticles combined with isoniazid unlike the use of isoniazid only or silver nanoparticles. When treating experimental tuberculosis, survival rates and histological tests of the lung tissue confirmed that the combination of isoniazid and silver nanoparticles was preferable compared to the single use of the above components.

Keywords

• множественная лекарственная устойчивость микобактерий туберкулеза
• изониазид