International Journal of General Medicine (Nov 2021)
Multidimensional Analysis of the Role of Charged Multivesicular Body Protein 7 in Pan-Cancer
Abstract
Yu Guo, Jian Shi, Zeyun Zhao, Min Wang Department of the General Surgery, Jilin University Second Hospital, Changchun, Jilin, People’s Republic of ChinaCorrespondence: Jian Shi; Min WangDepartment of the General Surgery, Jilin University Second Hospital, No. 218, Ziqiang Street, Nanguan District, Changchun, Jilin, People’s Republic of ChinaTel +86 18135435372; +86 15364759940Email [email protected]; [email protected]: Charged multivesicular body protein 7 is briefly referred to as CHMP7, and it plays a significant role in the endosomal sorting pathway. CHMP7 can form a complex with ESCRTIII to jointly complete the process of contraction, shear bud neck and final membrane shedding.Methods: TCGA, GEO and CPTAC were chosen for the analysis of the role of CHMP7 in pan-cancer. Role of CHMP7 in pan-cancer was analyzed using R software and tools such as TIMER, GEPIA, UALCAN, String and DiseaseMeth. It includes differential expression analysis of CHMP7, survival analysis, genetic variation analysis, DNA methylation analysis, post-translationally modified protein phosphorylation analysis and functional enrichment analysis.Results: CHMP7 presents low expression in the majority of tumor tissues and the prognosis is poor in the low expression group. The common gene mutation in CHMP7 is deep deletion, which may lead to frameshift mutations, resulting in a poor prognosis. Functional alterations due to DNA methylation and post-transcriptional protein modifications may be closely associated with tumors. GO analysis revealed that CHMP7-related genes are involved in the composition of the various ESCRT complexes. In terms of molecular function, they mainly bind to GTP, exert GTPase activity and promote multivesicular bodies assembly. In the KEGG enrichment analysis, the main pathways expressed by CHMP7 and related genes were endocytosis, gap junction and phagosome.Conclusion: Pan-cancer analysis showed that CHMP7 expression was statistically correlated with clinical prognosis, DNA methylation, protein phosphorylation and immune cell infiltration, which may provide new ideas or targets for the diagnosis or treatment.Keywords: pan-cancer, CHMP7, endosomal sorting complex required for transport, ESCRT, prognosis
